CMM546

040291

This course will cover the fundamentals and theory of Fluorescence Microscopy. Students will learn image formation theory based on optical theory and light interactions. Once the fundamentals have been covered, the class will discuss several modes of fluorescence microscopy, including: Wide-field fluorescence, Confocal microscopy, Convolution and deconvolution, Super-Resolution imaging. The content will conclude with a discussion of Imaging Ethics, as relates to fluorescence microscopy and as accepted by the world's scientific community. Fluorescence microscopy is a frequently used tool in biomedical research, and understanding of the method and results obtained from it is essential to understanding much of our knowledge of cellular and molecular mechanisms of the human body in health and disease. Fluorescence methods are also routinely used as diagnostic tools in medicine. Therefore, students pursuing many fields in medicine and biological research will benefit from an understanding of this technology.

GRD - Regular Grades A, B, C, D, E

Graduate

Successful completion of CMM 541.

CMM446